4P52
Crystal structure of homoserine kinase from Cytophaga hutchinsonii ATCC 33406, NYSGRC Target 032717.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-04 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9791 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 101.608, 101.608, 195.982 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.330 - 2.600 |
R-factor | 0.1741 |
Rwork | 0.172 |
R-free | 0.20900 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.474 |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
Rmerge | 0.141 | 0.045 | 0.769 |
Total number of observations | 219070 | ||
Number of reflections | 34842 | ||
<I/σ(I)> | 7.4 | ||
Completeness [%] | 99.9 | 98.5 | 100 |
Redundancy | 6.3 | 6.9 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | 0.2M Na2HPO4/KH2PO4, pH 6.2, 2.5M sodium chloride |