4P4P
Crystal structure of Leishmania infantum polymerase beta: Nick complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-04-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 106.497, 106.497, 159.609 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.303 - 2.297 |
R-factor | 0.1948 |
Rwork | 0.191 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bpy |
RMSD bond length | 0.006 |
RMSD bond angle | 1.045 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.297 |
Number of reflections | 24544 |
<I/σ(I)> | 20.9 |
Completeness [%] | 100.0 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6 and 12.5% PEG 4000 |