4P4M
Crystal structure of Leishmania infantum polymerase beta: Ternary P/T complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 108.680, 108.680, 151.641 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.945 - 1.919 |
| R-factor | 0.1653 |
| Rwork | 0.164 |
| R-free | 0.18150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bpy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.292 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.130 | 1.948 |
| High resolution limit [Å] | 1.918 | 1.918 |
| Number of reflections | 41119 | |
| <I/σ(I)> | 38.4 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 120 mM magnesium chloride, 0.4 M Tris-HCl, pH 7.5 |
