4P47
CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM OCHROBACTRUM ANTHROPI (Oant_4429), TARGET EFI-510151, C-TERMIUS BOUND IN LIGAND BINDING POCKET
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-17 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.183, 68.695, 76.431 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.935 - 1.300 |
R-factor | 0.1648 |
Rwork | 0.164 |
R-free | 0.18800 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.253 |
Data scaling software | SCALA (3.3.20) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.535 | 25.535 | 1.370 |
High resolution limit [Å] | 1.300 | 4.110 | 1.300 |
Rmerge | 0.054 | 0.782 | |
Rmeas | 0.116 | ||
Rpim | 0.043 | 0.023 | 0.331 |
Total number of observations | 493001 | 16126 | 61852 |
Number of reflections | 70496 | ||
<I/σ(I)> | 9.9 | 25 | 2.4 |
Completeness [%] | 99.6 | 97.6 | 98.6 |
Redundancy | 7 | 6.8 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | Protein (40.24 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT); Reservoir (0.2 M Ammonium Sulfate, 20 %(w/v) PEG 3350); Cryoprotection (20% ethylene glycol, 80% reservoir) |