4P3V

Crystal structure of the E. coli HU beta2 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98401
Spacegroup name	P 65 2 2
Unit cell lengths	51.107, 51.107, 110.462
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.260 - 1.250
R-factor	0.1565
Rwork	0.156
R-free	0.17340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mul
RMSD bond length	0.005
RMSD bond angle	0.888
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.260	1.270
High resolution limit [Å]	1.250	1.250
Rmerge	0.045	0.892
Number of reflections	24255
<I/σ(I)>	20.9	2.1
Completeness [%]	99.4	97.9
Redundancy	9.4	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	30% PEG800, 0.2M lithium sulfate, 0.1M sodium acetate