4P3U
Crystal structure of the bacterial A1408U-mutant ribosomal decoding site (C2 form 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.380, 32.730, 80.990 |
Unit cell angles | 90.00, 117.06, 90.00 |
Refinement procedure
Resolution | 100.000 - 3.000 |
Rwork | 0.295 |
R-free | 0.30240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.287 |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.600 | 25.600 | 3.110 |
High resolution limit [Å] | 3.000 | 6.430 | 3.000 |
Rmerge | 0.091 | 0.037 | 0.377 |
Total number of observations | 26787 | 2525 | 2635 |
Number of reflections | 7533 | ||
<I/σ(I)> | 7.1 | 24.3 | 2.2 |
Completeness [%] | 99.4 | 95.3 | 100 |
Redundancy | 3.53 | 3.21 | 3.57 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium Cacodylate, Spermine tetrahydrochloride, PEG3350, Sodium Cacodylate |