4P3T
Crystal structure of the bacterial A1408C-mutant ribosomal decoding site
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-30 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.91992, 0.92005, 0.90660, 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.437, 40.475, 41.272 |
Unit cell angles | 90.00, 103.07, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
Rwork | 0.248 |
R-free | 0.27060 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.881 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASES |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.200 | 40.200 | 1.660 |
High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
Rmerge | 0.039 | 0.026 | 0.295 |
Total number of observations | 102374 | 8105 | 10263 |
Number of reflections | 14089 | ||
<I/σ(I)> | 19.2 | 55.5 | 4.9 |
Completeness [%] | 98.4 | 87.4 | 100 |
Redundancy | 7.21 | 6.18 | 7.23 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium Cacodylate (pH7), Spermine tetrahydrochloride, MPD, Ammonium Chloride |