4P3J
Apo inward-facing state of the glutamate transporter homologue GltPh in alkali-free conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 106.948, 196.843, 207.484 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.500 |
| R-factor | 0.26395 |
| Rwork | 0.263 |
| R-free | 0.27845 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p19 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.116 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.881 | |
| Number of reflections | 50777 | |
| <I/σ(I)> | 13.7 | 1.24 |
| Completeness [%] | 94.4 | |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | PEG 400 Potassium Citrate Potassium Chloride Magnesium Chloride |
