4P3I
Structure of the P domain from a GI.7 Norovirus variant in complex with LeA HBGA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-12-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97935 |
Spacegroup name | P 1 |
Unit cell lengths | 62.168, 63.277, 90.400 |
Unit cell angles | 72.77, 82.20, 60.31 |
Refinement procedure
Resolution | 38.920 - 1.694 |
R-factor | 0.1562 |
Rwork | 0.155 |
R-free | 0.17740 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.461 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 1.694 |
Number of reflections | 122863 |
<I/σ(I)> | 17.3 |
Completeness [%] | 97.5 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M Sodium Formate 0.1M Bis Tris Propane 20% PEG3350 |