4P3G

Structure of the SRP68-RBD from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9919
Spacegroup name	P 21 21 21
Unit cell lengths	46.840, 95.050, 223.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.015 - 2.700
R-factor	0.2022
Rwork	0.200
R-free	0.25320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.241
Data reduction software	MOSFLM
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	58.690
High resolution limit [Å]	2.700
Number of reflections	28410
<I/σ(I)>	10.4
Completeness [%]	99.9
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	NaCl, PEG 3350