4P3C

MT1-MMP:Fab complex (Form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 2 21 21
Unit cell lengths	52.314, 80.975, 95.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.280 - 1.943
R-factor	0.1556
Rwork	0.152
R-free	0.20010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yec
RMSD bond length	0.007
RMSD bond angle	1.062
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1593))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.980
High resolution limit [Å]	1.943	5.290	1.950
Rmerge	0.109	0.049	0.427
Total number of observations	323825
Number of reflections	30586
<I/σ(I)>	8.2
Completeness [%]	100.0	100	100
Redundancy	10.6	9.7	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25% PEG 3350, 0.2M MgCl.hexahydrate, 0.1M Hepes