4P36
Crystal structure of DJ-1 With Zn(II) bound (crystal 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.015, 75.015, 75.525 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.840 - 1.182 |
R-factor | 0.1193 |
Rwork | 0.119 |
R-free | 0.13840 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.284 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.900 | 1.200 |
High resolution limit [Å] | 1.180 | 1.180 |
Rmerge | 0.070 | 0.650 |
Number of reflections | 80469 | |
<I/σ(I)> | 16.2 | 2.2 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 8.9 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 200 mM Sodium citrate 30% (v:v) PEG-400 500 uM Zinc sulfate |