4P32
Crystal structure of E. coli LptB in complex with ADP-magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.900, 36.020, 64.510 |
| Unit cell angles | 90.00, 96.16, 90.00 |
Refinement procedure
| Resolution | 31.799 - 1.550 |
| R-factor | 0.1986 |
| Rwork | 0.198 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p31 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.950 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 95.396 |
| High resolution limit [Å] | 1.544 |
| Number of reflections | 63324 |
| <I/σ(I)> | 9.8 |
| Completeness [%] | 98.6 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M MES pH 6.5, 30% PEG 4000 |
