4P2Z
Structure of NavMS T207A/F214A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.010, 333.040, 80.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.680 - 3.080 |
| R-factor | 0.2588 |
| Rwork | 0.258 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zjz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.700 | 3.300 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rmerge | 0.278 | 0.928 |
| Number of reflections | 18905 | |
| <I/σ(I)> | 8.4 | 1.6 |
| Completeness [%] | 93.6 | 71.1 |
| Redundancy | 5.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1M Tris-HCl, pH8, 34% v/v PEG400 |
