4P2K

Structure of Ephrin type-A receptor 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	54.885, 74.796, 76.019
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.248 - 1.499
R-factor	0.1737
Rwork	0.173
R-free	0.18980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.067
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.079	0.072	0.176
Total number of observations	358821
Number of reflections	50533
<I/σ(I)>	26.1
Completeness [%]	99.4	90.8	100
Redundancy	7.1	6.3	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2M Ammonium citrate tribasic pH7.0, 20% PEG3350