4P2I

Crystal structure of the mouse SNX19 PX domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2013
Detector	ADSC QUANTUM 210r
Spacegroup name	P 1 21 1
Unit cell lengths	63.840, 32.938, 81.966
Unit cell angles	90.00, 107.52, 90.00

Refinement procedure

Resolution	30.440 - 1.900
R-factor	0.2133
Rwork	0.211
R-free	0.24730
RMSD bond length	0.007
RMSD bond angle	0.983
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall
Low resolution limit [Å]	31.700
High resolution limit [Å]	1.900
Rmerge	0.104
Number of reflections	26154
<I/σ(I)>	15.1
Completeness [%]	99.9
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20% PEG3350, 0.25 M NaCl, 5% glycerol, 0.1 M Tris (pH 8)