4P25
Structure of the P domain from a GI.7 Norovirus variant in complex with LeY HBGA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-12-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97939 |
Spacegroup name | P 1 |
Unit cell lengths | 62.314, 63.124, 90.580 |
Unit cell angles | 72.80, 82.18, 60.85 |
Refinement procedure
Resolution | 38.996 - 1.499 |
R-factor | 0.1362 |
Rwork | 0.134 |
R-free | 0.17450 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.161 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 1.499 |
Number of reflections | 176762 |
<I/σ(I)> | 28.5 |
Completeness [%] | 96.8 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M Sodium Formate 0.1M Bis Tris Propane 20% PEG3350 |