4P1T
Crystal structure of the DBL3X-DBL4epsilon double domain from the extracellular part of VAR2CSA PfEMP1 from Plasmodium falciparum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-27 |
Detector | ADSC QUANTUM 1 |
Wavelength(s) | 0.98011 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 99.794, 129.336, 64.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.160 - 2.900 |
R-factor | 0.207 |
Rwork | 0.203 |
R-free | 0.27600 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.160 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.263 | 0.936 |
Number of reflections | 17568 | |
<I/σ(I)> | 6.8 | 0.8 |
Completeness [%] | 92.1 | 92.1 |
Redundancy | 6.8 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 18% PEG 3000, 100 mM Bis-Tris buffer, 300 mM sodium chloride |