4P1N
Crystal structure of Atg1-Atg13 complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-12-04 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0, 0.9791, 0.9794, 0.964 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.995, 96.622, 63.330 |
| Unit cell angles | 90.00, 93.15, 90.00 |
Refinement procedure
| Resolution | 45.770 - 2.200 |
| R-factor | 0.21333 |
| Rwork | 0.209 |
| R-free | 0.25493 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.368 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 31856 | |
| <I/σ(I)> | 11.4 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 15.3 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 12% PEG 3350, 0.2M ammonium acetate, 0.1M acetate buffer |
