4P1M

The structure of Escherichia coli ZapA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 61 2 2
Unit cell lengths	54.100, 54.100, 328.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.381 - 1.950
R-factor	0.2263
Rwork	0.224
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w2e
RMSD bond length	0.006
RMSD bond angle	0.996
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge		0.840
Number of reflections	22080
<I/σ(I)>	19.4	2.7
Completeness [%]	99.9	99.9
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	ammonium sulfate, lithium sulfate, sodium citrate.