4P19
Closed, apo inward-facing state of the glutamate transporter homologue GltPh
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.933, 201.812, 207.142 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.250 |
| R-factor | 0.22417 |
| Rwork | 0.222 |
| R-free | 0.25806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kbc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.680 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.310 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.886 | |
| Number of reflections | 70319 | |
| <I/σ(I)> | 12.3 | 1.23 |
| Completeness [%] | 98.7 | |
| Redundancy | 5.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | PEG 400, potassium chloride, potassium citrate, magnesium chloride |
