4P13

Medium chain acyl-CoA dehydrogenase, K304E mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-11-28
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	95.270, 102.590, 149.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.660 - 1.730
R-factor	0.146
Rwork	0.145
R-free	0.16300
RMSD bond length	0.010
RMSD bond angle	0.990
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.660
High resolution limit [Å]	1.720
Number of reflections	152953
<I/σ(I)>	19
Completeness [%]	100.0
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	8% PEG4000, 140mm Tris-acetate pH 8.0