4P0U
YhdE E33A p4 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-01 |
| Detector | RIGAKU |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 |
| Unit cell lengths | 71.066, 71.066, 78.884 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.750 - 2.360 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.622 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.050 | 2.350 |
| Rmerge | 0.051 | 0.026 | 0.192 |
| Total number of observations | 65762 | ||
| Number of reflections | 16184 | ||
| <I/σ(I)> | 17.3 | ||
| Completeness [%] | 98.4 | 99.9 | 84 |
| Redundancy | 4.1 | 4.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M Magnesium Sulfate 0.1M MES buffer 10%~15% PEG8000 |
