4P0T
Crystal structure of human centromere protein M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 3 |
| Unit cell lengths | 104.030, 104.030, 33.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.015 - 1.493 |
| R-factor | 0.1268 |
| Rwork | 0.124 |
| R-free | 0.16360 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.854 |
| Data reduction software | xia2 (0.3.3.1) |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.250 | 1.540 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.361 | |
| Number of reflections | 64606 | |
| <I/σ(I)> | 26.3 | 6.4 |
| Completeness [%] | 98.3 | 96.3 |
| Redundancy | 9.4 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 100 mM bicine pH 8.5, 11 % MPD, 8 mM spermidine |
