4P0Q
Crystal structure of Human Mus81-Eme1 in complex with 5'-flap DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2013-04-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 86.409, 228.377, 52.637 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.452 - 2.851 |
R-factor | 0.2446 |
Rwork | 0.243 |
R-free | 0.27610 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.732 |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.850 |
Number of reflections | 24832 |
<I/σ(I)> | 21.6 |
Completeness [%] | 98.8 |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | Tris, Ethanol |