4P0I
Structure of the PBP NocT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-10-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.860, 69.710, 74.380 |
Unit cell angles | 90.00, 117.86, 90.00 |
Refinement procedure
Resolution | 32.430 - 1.890 |
R-factor | 0.2065 |
Rwork | 0.205 |
R-free | 0.23650 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.800 |
High resolution limit [Å] | 1.890 |
Number of reflections | 43103 |
<I/σ(I)> | 22.43 |
Completeness [%] | 99.4 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2M SA, 0.1 M Na citrate pH 5.6, 0.2M K tartrate, 5% PEG400 |