4P0C
Crystal Structure of NHERF2 PDZ1 Domain in Complex with LPA2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.12719 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 26.360, 40.250, 37.100 |
Unit cell angles | 90.00, 107.42, 90.00 |
Refinement procedure
Resolution | 24.208 - 1.339 |
R-factor | 0.1466 |
Rwork | 0.145 |
R-free | 0.17690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 20CS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.191 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 24.210 |
High resolution limit [Å] | 1.170 |
Rmerge | 0.045 |
Number of reflections | 24020 |
<I/σ(I)> | 15.3 |
Completeness [%] | 95.8 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM sodium acetate, 0.2 M ammonium acetate |