4P09
Crystal structure of HOIP PUB domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2013-03-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 47.920, 47.920, 152.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.722 - 1.700 |
R-factor | 0.1836 |
Rwork | 0.181 |
R-free | 0.23030 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.802 |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.900 |
High resolution limit [Å] | 1.700 |
Number of reflections | 20365 |
<I/σ(I)> | 13.5 |
Completeness [%] | 99.0 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0,1M Tris, 20% 2-methyl-2,4-pentanediol, 15% Polyethyleneglycol 3350, pH 8.5 |