4OZW
Crystal Structure of the periplasmic alginate lyase AlgL H202A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.406, 59.437, 102.083 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.371 - 1.640 |
R-factor | 0.1691 |
Rwork | 0.168 |
R-free | 0.19190 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.971 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.700 |
High resolution limit [Å] | 1.640 | 3.530 | 1.640 |
Rmerge | 0.090 | 0.074 | 0.262 |
Total number of observations | 644419 | ||
Number of reflections | 42819 | ||
<I/σ(I)> | 20.6 | ||
Completeness [%] | 99.4 | 100 | 94 |
Redundancy | 15 | 19.2 | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 4.6, 26%(w/v) PEG 4000, 0.01 M Taurine |