4OZU

Crystal Structure of WD40 domain from Toxoplasma gondii coronin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	C 2 2 21
Unit cell lengths	55.130, 82.510, 156.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.839 - 1.650
R-factor	0.1647
Rwork	0.163
R-free	0.20230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aq5
RMSD bond length	0.005
RMSD bond angle	0.967
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	43134
<I/σ(I)>	12.2
Completeness [%]	99.2
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PMME 2000, ammonium acetate, TRIS-HCl