4OZG
D2 protein complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 266.024, 60.266, 138.235 |
| Unit cell angles | 90.00, 114.04, 90.00 |
Refinement procedure
| Resolution | 43.480 - 3.000 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.24090 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.480 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.074 |
| Number of reflections | 39276 |
| <I/σ(I)> | 8.1 |
| Completeness [%] | 96.5 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Mother liquor: 0.15- 0.25M CaOAc, 0.1 M Tris/HCl, 12-18% PEG3350, Additives: 2-3 mM reduced and oxidised Glutathione |
