4OZ7
Methanobactin production by methanotrophic bacteria and their structural diversity from Methylosinus strains: Insights into copper release
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-21 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.98 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 36.720, 39.420, 40.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.160 - 1.650 |
R-factor | 0.22216 |
Rwork | 0.221 |
R-free | 0.25070 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 2.859 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.160 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.087 | 0.342 |
Number of reflections | 3677 | |
<I/σ(I)> | 11.3 | 3.4 |
Completeness [%] | 99.0 | 94.1 |
Redundancy | 6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.1 M Sodium Malonate, 100 mM Hepes pH 7.0, 0.5% v/v Jeffamine ED-2001, 0.5% w/v Octyl-beta-D-glucoside, Crystal were cryoprotected with the above condition supplemented with 20% glycerol. |