4OYL
Humicola insolens cutinase in complex with mono-ethylphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.89 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.630, 66.400, 71.980 |
| Unit cell angles | 90.00, 119.30, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.050 |
| R-factor | 0.1455 |
| Rwork | 0.143 |
| R-free | 0.18355 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.683 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.115 | |
| Number of reflections | 36134 | |
| <I/σ(I)> | 10.5 | 2.6 |
| Completeness [%] | 97.4 | 89.3 |
| Redundancy | 2.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Protein concentration 30 mg mL-1, 0.1 M TRIS/HCl pH 8.5, 50 mM lysine, PEG MME 2K 11% v/v of 50% w/v stock solutions or PEG MME 550 16% v/v of 50 % w/v stock solutions |
