4OYK
Structure of HOIP PUB domain bound to OTULIN PIM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 61 |
Unit cell lengths | 64.049, 64.049, 172.022 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.468 - 2.000 |
R-factor | 0.2024 |
Rwork | 0.201 |
R-free | 0.23680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4oyj |
RMSD bond length | 0.002 |
RMSD bond angle | 0.613 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.470 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.129 | 0.604 |
Number of reflections | 26916 | |
<I/σ(I)> | 5.9 | 2 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 4.9 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 295 | 32% PEG 6000, 1M LiCl, 100 mM Tris pH 8.4 |