4OYI
Human solAC Complexed with (4-Amino-furazan-3-yl)-phenyl-methanone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 |
Unit cell lengths | 98.995, 98.995, 97.744 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.580 - 1.700 |
R-factor | 0.17154 |
Rwork | 0.170 |
R-free | 0.20745 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.515 |
Refinement software | REFMAC (5.8.0064) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.630 |
High resolution limit [Å] | 1.700 |
Number of reflections | 56679 |
<I/σ(I)> | 15.7 |
Completeness [%] | 95.1 |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1ul of protein solution was mixed with 1ul of reservoir solution (0.1M sodium acetate, pH 4.8, 0.2M trisodium citrate, 16-18% PEG4K and 10% glycerol) and left to equilibrate at 4C |