4OYC
Crystal structure of the PrgK periplasmic domain 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-22 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.190, 34.740, 64.030 |
| Unit cell angles | 90.00, 110.77, 90.00 |
Refinement procedure
| Resolution | 36.090 - 2.600 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yj7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.090 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 4992 |
| <I/σ(I)> | 4.5 |
| Completeness [%] | 99.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100 mM sodium acetate pH 5.5, 20 % PEG 6000, 50 mM NaCl, 50 mM MgCl2 |
