4OYB
Crystal Structure Analysis of the solAC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.03320 |
Spacegroup name | P 63 |
Unit cell lengths | 99.746, 99.746, 98.126 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.748 - 1.700 |
R-factor | 0.17212 |
Rwork | 0.170 |
R-free | 0.21083 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.483 |
Refinement software | REFMAC (5.8.0064) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 86.530 |
High resolution limit [Å] | 1.700 |
Number of reflections | 60896 |
<I/σ(I)> | 12.9 |
Completeness [%] | 99.4 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1ul of protein solution was mixed with 1ul of reservoir solution (0.1M sodium acetate, pH 4.8, 0.2M trisodium citrate, 16-18% PEG4K and 10% glycerol) and left to equilibrate at 4C |