4OX7

Structure of Synechococcus elongatus PCC 7942 CcmK2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9762
Spacegroup name	I 1 2 1
Unit cell lengths	85.789, 73.117, 104.183
Unit cell angles	90.00, 96.64, 90.00

Refinement procedure

Resolution	36.496 - 2.100
R-factor	0.1841
Rwork	0.181
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a10
RMSD bond length	0.004
RMSD bond angle	0.792
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.500	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.093	0.577
Number of reflections	31829
<I/σ(I)>	9.79	2.08
Completeness [%]	85.0	93.2
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	0.1 M HEPES pH 6.6. 50% MPD