4OX6
Structure of Synechococcus elongatus PCC 7942 CcmK4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9762 |
Spacegroup name | P 63 |
Unit cell lengths | 67.452, 67.452, 63.351 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.726 - 1.337 |
R-factor | 0.1682 |
Rwork | 0.167 |
R-free | 0.18940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a10 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.792 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.726 | 1.380 |
High resolution limit [Å] | 1.337 | 1.337 |
Rmerge | 0.045 | 0.723 |
Number of reflections | 34786 | |
<I/σ(I)> | 28.33 | 1.63 |
Completeness [%] | 93.8 | 63.4 |
Redundancy | 9.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MgCl2, 0.1 M sodium citrate pH 5.0, 15 % (w/v) PEG 4000 |