4OX5
Structure of the LdcB LD-carboxypeptidase reveals the molecular basis of peptidoglycan recognition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-28 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 48.855, 60.414, 138.269 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.949 - 1.800 |
R-factor | 0.1933 |
Rwork | 0.191 |
R-free | 0.25147 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 2.003 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.950 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 25656 | |
<I/σ(I)> | 34.4 | 2.4 |
Completeness [%] | 98.6 | 86.9 |
Redundancy | 24.3 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1 M mixture of amino acids (glycine, L-glutamate, and racemates of alanine, serine and lysine), 0.1 M buffer system 2, pH 7.5 and 37.5 % MPD/PEG1000/PEG3350 |