4OWX
Structural basis of SOSS1 in complex with a 12nt ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.630, 162.520, 65.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.807 - 2.300 |
R-factor | 0.1846 |
Rwork | 0.182 |
R-free | 0.22580 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.119 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.810 |
High resolution limit [Å] | 2.300 |
Number of reflections | 47747 |
<I/σ(I)> | 11.4 |
Completeness [%] | 99.8 |
Redundancy | 7.78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 16% PEG3350, 0.1M MES pH6.0, 0.1M ammonium formate |