4OWS
Anthranilate phosphoribosyl transferase from Mycobacterium tuberculosis in complex with 4-methylanthranilate, PRPP and Magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.726, 91.780, 120.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.030 - 2.430 |
| R-factor | 0.1811 |
| Rwork | 0.179 |
| R-free | 0.22730 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.469 |
| Data scaling software | SCALA (3.3.15) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 120.567 | 120.567 | 2.560 |
| High resolution limit [Å] | 2.426 | 7.670 | 2.430 |
| Rmerge | 0.024 | 0.495 | |
| Rmeas | 0.102 | ||
| Rpim | 0.037 | 0.011 | 0.209 |
| Total number of observations | 247716 | 7987 | 36407 |
| Number of reflections | 34257 | ||
| <I/σ(I)> | 17.6 | 50.4 | 3.9 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 7.2 | 6.6 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | imidazole/malate, PEG 4000 |
