4OWP
Crystal structure of rpn11 in a heterodimer complex with rpn8, representing the active portion of the proteasome lid.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-01-21 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 70.388, 70.388, 199.648 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.763 - 2.350 |
R-factor | 0.191 |
Rwork | 0.188 |
R-free | 0.24210 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.077 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.5) |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.390 |
High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
Rmerge | 0.138 | 0.057 | 0.810 |
Total number of observations | 362134 | ||
Number of reflections | 21697 | ||
<I/σ(I)> | 8.2 | 1.72 | |
Completeness [%] | 98.6 | 98.7 | 88.4 |
Redundancy | 16.7 | 15.1 | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10% PEG 5000, 5% Tacsimate, 0.1 M Hepes pH 7.0 |