4OWG
Crystal structure of rabbit muscle triosephosphate isomerase-PEP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.848, 72.071, 161.204 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.080 - 1.550 |
R-factor | 0.169 |
Rwork | 0.167 |
R-free | 0.20300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r2r |
RMSD bond length | 0.020 |
RMSD bond angle | 1.905 |
Data reduction software | MOSFLM (7.0.9) |
Phasing software | PHASER (2.5.5) |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.080 |
High resolution limit [Å] | 1.550 |
Number of reflections | 59113 |
<I/σ(I)> | 8 |
Completeness [%] | 93.4 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH: 6.5, 25% PEG 8000 |