4OWD
Crystal structure of MltF from Pseudomonas aeruginosa complexed with cysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2012-03-03 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.542 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.601, 82.618, 96.235 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.760 - 2.210 |
R-factor | 0.1943 |
Rwork | 0.192 |
R-free | 0.23820 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.909 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.686 | 2.320 |
High resolution limit [Å] | 1.878 | 2.210 |
Rmerge | 0.040 | 0.079 |
Rmeas | 0.045 | |
Rpim | 0.020 | |
Total number of observations | 95983 | |
Number of reflections | 95977 | |
<I/σ(I)> | 22.7 | 11.4 |
Completeness [%] | 93.2 | 94.9 |
Redundancy | 4.3 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Tris pH 8.0, 0.2 M MgCl2, 20% (w/v) PEG8000 |