4OW5
Structural basis for the enhancement of virulence by entomopoxvirus fusolin and its in vivo crystallization into viral spindles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.270, 71.270, 129.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.600 - 1.900 |
| R-factor | 0.1712 |
| Rwork | 0.168 |
| R-free | 0.20250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bem |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 1.980 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.265 | 0.863 |
| Number of reflections | 26941 | |
| <I/σ(I)> | 10.6 | 1.9 |
| Completeness [%] | 98.5 | 98.52 |
| Redundancy | 13.9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | in vivo crystallization | 301 | crystals are obtained in vivo |
