4OVX

Crystal structure of Xylose isomerase domain protein from Planctomyces limnophilus DSM 3776

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 43 21 2
Unit cell lengths	88.305, 88.305, 132.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.855 - 2.253
R-factor	0.2005
Rwork	0.198
R-free	0.24400
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.156
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.250	6.100	2.250
Rmerge	0.098	0.042	0.996
Total number of observations	296247
Number of reflections	25577
<I/σ(I)>	7
Completeness [%]	100.0	99.4	100
Redundancy	11.6	10.4	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.2 M Lithium Chloride, 20% PEG 3350