4OVJ
Extracellular solute-binding protein family 1 from Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.716, 38.819, 82.456 |
| Unit cell angles | 90.00, 92.15, 90.00 |
Refinement procedure
| Resolution | 34.540 - 1.650 |
| R-factor | 0.1212 |
| Rwork | 0.119 |
| R-free | 0.16290 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-3000 (3.11) |
| Phasing software | HKL-3000 (3.11) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.071 | 0.504 |
| Number of reflections | 45731 | |
| <I/σ(I)> | 23.8 | 2.23 |
| Completeness [%] | 99.5 | 94.1 |
| Redundancy | 6.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 0.2 M Li Sulfate, 0.1 M HEPES , 25% w/v PEG 3350 |
