4OVA
Structure of the two tandem Tudor domains and a new identified KH0 domain from human Fragile X Mental Retardation Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 130 |
Detector technology | CCD |
Collection date | 2013-12-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 232.643, 60.842, 168.856 |
Unit cell angles | 90.00, 122.99, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.2207 |
Rwork | 0.219 |
R-free | 0.25860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o8v |
RMSD bond length | 0.009 |
RMSD bond angle | 1.358 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 38927 | |
<I/σ(I)> | 2.4 | |
Completeness [%] | 99.0 | 99.9 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |