4OV1
The crystal structure of a novel electron transfer ferredoxin from R. palustris HaA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-25 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.362, 26.810, 91.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.111 - 2.306 |
| R-factor | 0.2247 |
| Rwork | 0.223 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z8q |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.503 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.074 | 0.126 |
| Number of reflections | 2832 | |
| <I/σ(I)> | 19.3 | 12.3 |
| Completeness [%] | 96.4 | 80.8 |
| Redundancy | 4.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.3M potassium sodium tartrate tetrahydrate, 0.1M Tris pH 8.5, 2.4M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
